SEPARATING METHOD OF SIALYLLACTOSE

摘要

PURPOSE: To provide a method to separate sialyllactoses with mutually difference bond patterns. CONSTITUTION: A raw material solution containig sialyllactose is supplied to a pseudofluidizing-bed type chromatographic separation apparatus (SMB) to separate an SAα2→3Galβ1→4Glc type havingα2→3 bond between sialic acid(SA) and lactose (Galβ1→4Glc) and an SAα2→6Galβ1→4Glc type havingα2→6 bond between them. Consequently, large quantities of the SAα2→3Galβ1→4Glc and the SAα2→6Galβ1→4Glc can easily be separated, thus both the former and the latter can be produced in an industrial scale.