发明名称 |
STRAIN OF HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS L - PRODUCER OF MONOCLONAL ANTIBODIES TO SURFACE ANTIGEN A-DETERMINANT OF HEPATITIS B VIRUS |
摘要 |
FIELD: molecular biology, virology. SUBSTANCE: strain of hybrid cultured cells HBSV-3 is obtained by fusion of myeloma P3-NS1-Ag4-1 cells with murine splenocytes (strain BALB/c) immunized with HBAg using polyethylene glycol (molecular mass = 4000). Hybridoma HBSV-3 produces monoclonal antibodies IgG1 that interact specifically with a-determinant HBAg. Strain of hybrid cells grows as stationary suspension and induces ascite tumor in mice BALB/c. Titer values of monoclonal antibodies produced by hybridoma are 1x10and 1x10in cultural and ascite fluid, respectively. Strain is deposited in All-Union Collection of cell cultures at number "ВСКК(П) 627 Д". EFFECT: preparing strain indicated above. 1 dwg |
申请公布号 |
RU94038730(A) |
申请公布日期 |
1996.09.27 |
申请号 |
RU19940038730 |
申请日期 |
1994.10.12 |
申请人 |
AKTSIONERNOE OBSHCHESTVO OTKRYTOGO TIPA "DIAVEKT LTD" |
发明人 |
AMMOSOV A.D.;RUKAVISHNIKOV M.JU.;PORYVAEVA V.A. |
分类号 |
C12N5/18;C12P21/08 |
主分类号 |
C12N5/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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