Mitochondrial P450

摘要

The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome. An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5 beta -cholestane-3 alpha , 7 alpha , 12 alpha , 27-tetrol, 25-hydroxyvitamin D3 and 1 alpha ,25-dihydroxyvitamin D3 are also disclosed.