METHOD OF DIFFERENTIAL DIAGNOSIS OF DIABETIC GLOMERULOSCLEROSIS AND CHRONIC GLOMERULONEPHRITIS

摘要

FIELD: medicine, clinical biochemistry. SUBSTANCE: method involves determination of plasminogen concentration in nephrological patient blood plasma. Plateletless blood plasma is diluted by two-fold with sodium-phosphate buffer, pH = 7.4, the obtained mixture is applied on the column filled with lysine-agarose and biospecifically substrate-bound plasminogen is eluted with 0.1M epsilon-aminocaproic acid solution. Eluate extinction is measured by spectrophotometrically and plasminogen concentration in plasma is calculated by extinction value. Diabetic glomerulosclerosis is diagnosed at plasminogen concentration 0.187 mg/l and above and chronic glomerulonephritis at concentration 0.150 mg/l and less. EFFECT: improved method of differential diagnosis.