DETECTING DNA DAMAGE, MEASURING DNA REPAIR RATES, AND MONITORING DNA REPAIR ENZYME ACTIVITY

摘要

The present invention includes an improved method to detect and quantitatively assay the amount of DNA damage in large double-stranded DNA templates starting with less than 50 ng of sample DNA. This invention has utility in vivo for quantitating gene-specific DNA damage, measuring DNA repair rates and monitoring efficacy of anti-neoplasia therapy in patients. Additionally, this invention is useful to detect and assess risk due to mutagenic environmental hazard site, and monitor hazard site dynamics and includes a device for accomplishing these objects.