Purified thermococcus barossii dna polymerase

摘要

A preparation of a purified DNA polymerase substantially identical to native DNA polymerase isolated from Thermococcus barossii is disclosed. In a preferable form of the present invention, the DNA polymerase is approximately 68 kDa in size, retains at least 90% activity after 8 hours of incubation at 95 DEG C. and retains between 60% and 80% proof-reading ability when catalyzing a polymerization reaction with a primer with a 3 base pair mismatch.