SCREENING FOR MODULATORS OF BIOMOLECULES

摘要

This invention provides methods for screening for a modulator of a biomolecule by comparing the effects of a putative modulator on a phenotypic sensor in one or more conditional growth cell types having altered biomolecules with the effect on that sensor in a cell type in which those biomolecules are normal. The altered biomolecule or the biochemical pathways related to that biomolecule result in differential effects, and/or in phenotypic effect which can be essentially reproduced in the normal cell by the effects of the modulator. Also provided are methods for screening for a modulator and for characterizing and evaluating a modulator by comparing the effects of the modulator in a plurality of cells having different altered biomolecules and/or on a plurality of different phenotypic sensors. The methods are also applicable for characterizing novel modulators with reference to known modulators, such as to identifying a mechanism of action or characterizing the molecular interactions of the modulator.