发明名称 |
Nucleic acid encoding synthesis-deficient thermostable DNA polymerase |
摘要 |
A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. Cleaving enzymes are produced through the use of novel DNA sequences which encode novel thermostable polymerases.
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申请公布号 |
US5541311(A) |
申请公布日期 |
1996.07.30 |
申请号 |
US19930073384 |
申请日期 |
1993.06.04 |
申请人 |
THIRD WAVE TECHNOLOGIES, INC. |
发明人 |
DAHLBERG, JAMES E.;LYAMICHEV, VICTOR I.;BROW, MARY ANN D. |
分类号 |
C12N1/21;C07H21/00;C07K14/47;C12N9/12;C12N9/14;C12N9/16;C12N15/09;C12N15/10;C12N15/113;C12N15/55;C12Q1/44;C12Q1/68;C12Q1/70;C12R1/19;G01N33/53;(IPC1-7):C12N1/21;C12N15/00;C07H21/04 |
主分类号 |
C12N1/21 |
代理机构 |
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地址 |
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