| 摘要 |
Sandwich type immunoassay of cardiac troponine I (I) uses 2 monoclonal antibodies (Ab) against (I), 1 being labelled with an enzyme. The Ab have, naturally or by cooperation, an equilibrium dissociation constant for (I) <= 10<-8> (pref. 10<-9>) M. A chemiluminescent substrate (II), derived from a diacylhydrazine or 1,2-dioxetane, is used for enzymatic detection. Also claimed is a kit for carrying out the above assay.
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