Process for the preparation of pyruvic acid using permeabilized transformants of H. polymorha and P. pastoris which express glycolate oxidase and catalase

摘要

A process for the production of pyruvic acid involving the enzymatic reaction of L-lactic acid and oxygen in an aqueous solution in the presence of catalysts glycolate oxidase ((S)-2-hydroxy-acid oxidase, EC 1.1.3.15) and catalase (EC 1.11.1.6). High yields of pyruvate at high purity can be achieved at commercially acceptable concentration without substantial product inhibition of the enzyme.