| 摘要 |
<p>The present invention provides compositions, methods and kits for detection and quantitation of pathogenic organisms. The composition of the invention is an oligonucleotide probe comprising a bacteriophage covalently linked to one site on an oligonucleotide probe complementary to a conserved region of a pathogenic organism. At a second site, the oligonucleotide probe is linked to a matrix. The oligonucleotide probe contains a region complementary to one strand of a restriction endonuclease recognition site or an oligoribonucleotide moiety. The number of pathogenic organisms present in a biological fluid sample may be quantitated in accordance with the method of the invention by combining the composition of the invention with the sample, allowing hybridization to occur. Hybridization generates the intact restriction endonuclease site or a DNA-RNA hybrid, and by adding the appropriate restriction endonuclease or a nucleolytic enzyme capable of cleaving DNA-RNA hybrids, bacteriophage will be released for measurement. The kit of the invention provides components which allow the method of the invention to be performed.</p> |
