| 摘要 |
PCT No. PCT/FR92/00615 Sec. 371 Date Apr. 30, 1993 Sec. 102(e) Date Apr. 30, 1993 PCT Filed Jul. 1, 1992 PCT Pub. No. WO93/00863 PCT Pub. Date Jan. 21, 1993.A fertilization method wherein an oocyte puncture is performed and one or more oocytes are inserted into a container containing a culture medium having no added air or CO2 and intended for the fertilization and culture of the oocyte(s), as well as spermatozoa, whereafter the container is sealed and inserted into the vagina or uterus to allow fertilization of the oocyte(s) and culture of the embryo(s). The method is characterized in that it is performed without stimulating the cycle, with LH supervision by means of blood assays, and by programming the oocyte puncture according to the LH value. The container may be kept at a temperature of approximately 37 DEG C. for 2 to 6 hours for maturation before being inserted into the vagina.
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