A novel antileishmanial formulation containing compounds belonging to the series of (2Z,2'Z)-3,3'-(alkane-1,2-diylbis(azanediyl))bis(6-hydroxy-1- aryl/fluorenyl hex-2-en-1-ones) or their salts thereof showing antileishmanial activity under in vivo conditions against L. donovani in hamster model. These compounds or their formulation elicit anitleshmanial activity both via oral and ip routes.