发明名称 |
OXIDATION OF GLYCOLIC ACID TO GLYOXYLIC ACID USING A MICROBIAL CELL TRANSFORMANT AS CATALYST |
摘要 |
A process for the production of Glyoxylic acid from glycolic acid where an aqueous solution of about 200 to 2500 mM glycolic acid at a pH of 7 to 10, at 0 DEG to 40 DEG C is contacted with an effective amount of whole microbial cells (e.g., Aspergillus nidulans, Pichia pastoris, Hansenula polymorpha and Escherichia coli) which as a result of genetic engineering expresses the enzyme glycolate oxidase, (S)-2-hydroxy-acid oxidase (EC 1.1.3.15), to achieve an effective concentration range of 0.01 to about 100 IU/mL and optionally expresses an endogenous catalase (EC 1.11.1.6). |
申请公布号 |
EP0621900(B1) |
申请公布日期 |
1996.06.26 |
申请号 |
EP19930902955 |
申请日期 |
1993.01.06 |
申请人 |
E.I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
ANTON, DAVID, LEROY;DICOSIMO, ROBERT |
分类号 |
C12N9/04;C12N9/08;C12N15/53;C12P7/40;C12R1/19;C12R1/66;C12R1/78;C12R1/84 |
主分类号 |
C12N9/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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