Procedure for preparing salmon calcitonin

摘要

A procedure for the preparation of salmon calcitonin comprising the condensation of fragment 1(SEQ ID NO: 1), a docosapeptide corresponding to the carboxamide end of the salmon calcitonin sequence conveniently protected and anchored on resin, with fragment 2(SEQ ID NO:2), a decapeptide corresponding to the amino end of the salmon calcitonin sequence conveniently protected and with a disulphide bridge ready formed between the two cysteines, and the treatment of the complete peptide skeleton (fragment 6 (SEQ ID NO:6)) with an acid to liberate the totally deprotected peptide from the resin. Lys(Boc)-Leu-Ser(tBu)-Gln-Glu(tBu)-Leu-His(Trt)- Lys(Boc)-Leu-Gln-Thr(tBu)-Tyr(tBu)-Pro-Arg(Pmc)- +TR Fragment 2 <IMAGE> -Leu-Gly-Lys(Boc)-Leu-Ser(tBu)-Gln-Glu(tBu)-Leu- +TR -His(Trt)-Lys(Boc) Leu-Gln-Thr(tBu)-Tyr(tBu)-Pro-Arg(Pmc)-Thr(tBu)- +TR