发明名称 |
Covalent dimer of kit ligand |
摘要 |
A modified form of KL, the ligand for the c-Kit proto-oncogene, has been prepared wherein the protein is stabilized by an intermolecular covalent linkage. The protein can be prepared by expression of a recombinant protein which is dissolved in denaturant and refolded under conditions resulting in a disulfide linked dimer. Examples demonstrate the purification and characterization of this disulfide-linked cysteine dimer kit ligand (KL-CD) which contains at least one intermolecular disulfide bond and has at least ten-fold greater activity in promoting cell proliferation than native, non-covalently linked KL, as measured in in vitro assays.
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申请公布号 |
US5525708(A) |
申请公布日期 |
1996.06.11 |
申请号 |
US19940220379 |
申请日期 |
1994.03.28 |
申请人 |
CYTOMED, INC. |
发明人 |
NOCKA, KARL H.;LOBELL, ROBERT B. |
分类号 |
A01K67/027;A61K38/00;A61K39/395;A61P7/06;A61P37/08;A61P43/00;C07H21/04;C07K14/475;C07K14/52;C07K19/00;C12N1/21;C12N15/02;C12N15/12;C12P21/02;C12R1/19;(IPC1-7):C07K14/52 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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