HETEROGENOUS SPECIFIC BOND TEST USED FOR DETECTION OR CONTENT DETERMINATION OF A GIVEN SUBSTANCE IN A SAMPLE ANALYSED, METHOD OF REDUCING NON-SPECIFIC BONDING IN PSEUDOHOMOGENOUS TESTS AND TESTING SET FOR PERFORMING IMMUNOLOGICAL TEST AND THOSE EMPLOYING GENETIC PROBE

摘要

A sensitive assay method has been discovered that reduces the amount of non-specific binding present in an assay, the method comprising detecting an analyte present in a sample through a specific binding reaction in which either an analogue of the analyte or a specific binding partner of the analyte is immobilized on a solid-phase and said specific binding reaction produces a detectable product immobilized on said solid-phase that may be correlated to the amount of analyte present in the sample wherein said assay employs an effective amount of a surfactant which is a polyoxyethylene-alkylether to reduce non-specific binding.