Chimeric receptors for the generation of selectively-activatable th-independent cytotoxic t cells

摘要

PCT No. PCT/US95/14171 Sec. 371 Date Nov. 1, 1995 Sec. 102(e) Date Nov. 1, 1995 PCT Filed Nov. 1, 1995 PCT Pub. No. WO96/13584 PCT Pub. Date May 9, 1996The invention provides cytotoxic T lymphocytes that express a chimeric cell surface receptor. The chimeric receptor enables the lymphocytes to become activated and to proliferate (via a TH-independent autocrine cycle) in response to adhesion between the lymphocyte and an antigen displaying cell (ADC) that might not normally activate the T cell. The chimeric receptors of the present invention comprise fusion polypeptides of the following structure:XC-TM-IC28wherein (XC) is an extracellular region derived from a receptor for a ligand that is expressed on the surface of an antigen-displaying cell, fused via a transmembrane region is (TM) to an intracellular region (IC28) which is derived from the intracellular region of the CD28 receptor.