发明名称 |
RECOMBINANT ADENOVIRUS AND METHODS OF USE THEREOF |
摘要 |
A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.
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申请公布号 |
CA2203809(A1) |
申请公布日期 |
1996.05.09 |
申请号 |
CA19952203809 |
申请日期 |
1995.10.27 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA |
发明人 |
FISHER, KRISHNA J.;WEITZMAN, MATTHEW;WILSON, JAMES M.;CHEN, SHU-JEN |
分类号 |
C12N15/09;A61K35/76;A61K48/00;C12N7/00;C12N15/861;(IPC1-7):C12N7/01;C12N7/04;C12N15/86 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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