| 摘要 |
Doubled-stranded oligonucleotide molecules for use in providing enchaned sequencing of DNA, comprising: a first stand having 1) a priming site; 2) a labeling region contiguous with the priming site, wherein the labeling region consists essentially of at least six identical nucleotides; and 3) a cohesive end contiguous with the labeling region, having a nucleotide sequence complementary to a nucleotide sequence generated by the action of a restriction enzyme; and a second strand annealed to the labeling region of the first stand, but not overlapping with the cohesive end. A method of DNA gene sequencing, involving the use of mobile priming sites for DNA polymerases in DNA sequencing, is also disclosed.
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