| 摘要 |
<p>According to the present invention, a process for the preparation of cyclosporin A comprising the steps of adding lower alkanol solvent to a culture fluid of a mutant of Tolypocladium inflatum and extracting the mixture; transferring the extract to a halogenated lower hydrocarbon solvent; if required, the resultant solution being treated with activated charcoal; and the solution being column chromatographed on silica gel is provided. The process of the present invention is advantageous from the aspect of process economics and environmental protection because the culture fluid is directly extracted without filtration, drying procedure is omitted and the chromatography process is simplified to a single process using silica gel.</p> |
