| 摘要 |
Cells, stained with a fluorescent dye taken up by DNA in individual cells, are scanned with a cytometer. The integrated values of all the cells are compiled to create a histogram of cell counts versus integrated fluorescent light (fig. 2), representing populations of cells having a complement of DNA, but not in the process of division (G0 phase), cells having two full complements of DNA, but which have not actually divided into two cells (G2 phase) and cells in the process of replicating their DNA (S phase). Errors, resulting from statistical errors, focusing problems, inaccurate measurement of background, etc., in the integrated values and the histogram, and affecting the accuracy and prognostic value of the peaks and separation phases, are corrected by modeling the convolution of the error function with the signal function, and removing the error function, by deconvolution, from the G0 and G2 peaks and the S phase (fig. 14).
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