The oligosaccharide structure of a ligand for e and p selectin

摘要

A specific ligand for E- and P-selectin was isolated from in vivo[<3>H]-glucosamine labeled HL-60 cells by a combination of weat germ agglutinin agarose and E- or P-selectin rIg-agarose chromatography. The total O-linked oligosaccharides on the purified molecule were released by alkaline hydrolysis in the presence of NaBH4 and analyzed by ion-exchange, size-exclusion and paper chromatography in combination with specific exoglycosidase digestions. Various derived ligands and carbohydrates are claimed.