| 摘要 |
The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharid es using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferases locus of Neisseria gonorrhoeae containing eve open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta.1~4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1~3 to Gal; and adding Gal .alpha.1~4 to Gal. The glycosyltransferascs of the invention are particularly suited to the synthes is of the oligosaccharides Gal.beta.1~4GlcNAc.beta.1~3Ga1~1~4Glc (a mimic of lacto-N- neotetraose), GalNAc.beta.1~3Gal.beta.1~4GleNAc.beta.1~3G.beta.1~4Glc.beta.1 ~4 (a mimic ganglioside), and Gal.alpha.1~4Gal.beta.1~4Gi.beta.1~4Hep~R (a mimic of the saccharide portion of globo-glycolipids). |
