| 摘要 |
Chimeric oligonucleotides for obtaining transcripts of and/or amplifying a target nucleic acid sequence comprise (in the 5' to 3' direction): (1) a DNA segment comprising a sense sequence of a RNA polymerase promoter, (2) a DNA segment (pref. 2-30. esp. 4-20 nucleotides long) comprising the transcription initiation site for the promoter, where at least the 3'-terminal region of this fragment is capable of hybridising with at least part of a 3'-terminal downstream sequence of the target, and (3) a 3'-blocked RNA segment (pref. 2-30. esp. 4-20 nucleotides long) whose 5'-terminal region is capable of hybridising with a portion of the target sequence contiguous to the portion of the downstream sequence to which segment (2) hybridises. Also claimed is a process for obtaining transcripts of a target nucleic acid sequence having non-overlapping 5'-terminal upstream and 3'-terminal downstream sequences, using a chimeric oligonucleotide as above (e.g. see figure).
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