| 摘要 |
<p>PURPOSE: To massively obtain the taxol exhibiting excellent therapeutic effects for critical ovarian cancer or pulmonary cancer patients in a high yield by culturing a callus induced from a taxol-containing plant in the presence of a chito oligosaccharide. CONSTITUTION: The method for producing the taxol comprises sterilizing the cut pieces of the stems or leaves of a taxol-containing plant (e.g. Taxus), planting the sterilized cut pieces in a culture medium containing 2,4- dichlorophenoxyacetic acid and kinetin, inducing the cut pieces into calluses in a dark place a 25 deg.C, transplanting the calluses in a culture medium containing a chito oligosaccharide in an amount of 0.25-10mg per g of the fresh calluses, culturing the calluses for 15 days without subjecting to a subculture operation, lyophilizing the cultured calluses, extracting the lyophilized product with a solvent comprising methanol:water (1:1, v/v) at room temperature, vacuum- concentrating the extraction solution, further extracting the extract with dichloromethane:water (1: 1, v/v), and subsequently subjecting the dichloromethane-soluble fraction to a chromatography, thus obtaining the objective taxol useful as an anticancer substance in a large amount and in a high yield.</p> |
