发明名称 |
ROLLER BOTTLE FOR TRANS-MEMBRANE CO-CULTURE OF CELLS AND METHOD FOR ITS USE |
摘要 |
A roller bottle for trans-membrane co-culture of cells includes an exterior receptacle with a longitudinal axis having a first chamber surrounded by a sidewall. The exterior receptacle has a first neck at one end having an opening therethrough providing fluid access to said first chamber. The roller bottle further includes an interior container with a longitudinal axis and a second chamber, the interior container being located coaxially within the exterior receptacle. The interior container has a second neck at one end providing fluid access to the second chamber. The exterior receptacle is formed from a material that is substantially impermeable to gas and liquid and is sealed in a substantially fluid tight fashion forming the first chamber that has fluid access by the first neck. At least a portion of the interior container is formed from a microporous material. The roller bottle of the invention enables culturing one cell type in the presence of another cell type for the study of interactions between the cells while maintaing a physical separation between the cell types providing a scale up in cell population size from well type inserts. The microporous membrane allows free interchange of media soluble cellular products between the cell types, but maintains physical separation.
|
申请公布号 |
CA2156578(A1) |
申请公布日期 |
1996.03.13 |
申请号 |
CA19952156578 |
申请日期 |
1995.08.21 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
MUSSI, EDWARD F.;GRAY, HARRY E. |
分类号 |
C12M1/24;C12M3/00;C12M3/04;C12M3/06;(IPC1-7):C12N5/02 |
主分类号 |
C12M1/24 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|