发明名称 |
Methods and compositions for the expression of a bone and prostate derived growth factor |
摘要 |
The role of tumor cell-host stromal interaction and stromal-specific growth factors in prostate cancer growth, progression and metastasis to the axial skeleton were investigated. A bone and prostate-derived growth factor (BPGF-1) was identified and cloned from an expression cDNA library prepared from human bone stromal cells. The cloned BPGF-1 cDNA comprised 3171 nucleotides with a single open reading frame of 1620 nucleotides. The BPGF-1 encodes two transcripts (3.3 and 2.5 kb) with approximately equal intensity. Polyclonal antibodies generated from the synthetic peptides that correspond to the nucleotide sequences of the cloned BPGF-1 cDNA reacted with a single putative BPGF-1 protein with an apparent molecular weight of 70 kDa. Southern blot analysis of human genomic DNA revealed that there is a single copy of BPGF-1 gene of 15 kb in size within BamHI sites. The cDNA for BPGF-1 encodes a protein that stimulates the proliferation of prostatic epithelial cells. The BPGF-1 gene is expressed predominantly in bone, prostate and seminal vesicles, with substantially higher expression in the bone than that of the prostate and seminal vesicles. |
申请公布号 |
AU3141495(A) |
申请公布日期 |
1996.03.04 |
申请号 |
AU19950031414 |
申请日期 |
1995.07.20 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
LELAND W CHUNG;CHUAN GAO |
分类号 |
A61K39/00;C07K14/475;C07K16/22;C12N15/18 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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