Biospecific determination method

摘要

The invention relates to a biospecific multiparametric determination method, in which a mixture of pre-produced microparticles, classified into different categories and representing different analytes, is used. Microparticles of different categories are coated with different biospecific reagents, which bind different analytes, and the microparticles contain one or more different fluorescent tetrapyrrole impurities in one or more concentrations depending upon the microparticle category. The strength of the fluorescence emission of the tetrapyrrole impurities indicates the category of the microparticle and the luminescence emission indicates the quantity of analyte bound to the microparticle.