The invention relates to a method of producing single-chain Fv molecules in eukaryotic cells, and to secretable sFv proteins having at least one nonnaturally occurring glycosylation site. The single-chain Fv molecules produced by this method are biologically active and capable of being secreted from eukaryotic cells into the cell culture medium.
申请公布号
CA2197232(A1)
申请公布日期
1996.02.22
申请号
CA19952197232
申请日期
1995.08.14
申请人
CREATIVE BIOMOLECULES, INC.;UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES (THE)
发明人
JOST, CAROLINA R.;SEGAL, DAVID M.;HUSTON, JAMES S.
