Method for ligation of an enzymatic nucleic acid molecule having a hammerhead motif to a separate nucleic acid molecule. The method includes use of ligation enhancing conditions in which ligation is enhanced at least two fold over than observed in a medium containing only Mg<2+> as a divalent cation at 20 DEG C.
申请公布号
WO9602651(A1)
申请公布日期
1996.02.01
申请号
WO1995US08327
申请日期
1995.06.29
申请人
RIBOZYME PHARMACEUTICALS, INC.
发明人
LONG, DAVID, M.;UHLENBECK, OLKE, C.;HERTEL, KLEMENS, J.
