发明名称 |
Ribozymes with optimized hybridizing arms, stems, and loops, trna embedded ribozymes and compositions thereof |
摘要 |
This invention is directed to improved catalytic compounds, hammerhead ribozymes, capable of hybridizing with a target RNA to be cleaved. These improved compounds have optimized stems (X)m * (X)m', loops (X)b and hybridizing arms. The invention is also directed to compositions for enhanced RNA cleavage which comprise a first synthetic non-naturally occurring oligonucleotide compound which comprises nucleotides whose sequence defines a conserved catalytic region and nucleotides whose sequence is capable of hybridizing with a predetermined target sequence and a second synthetic non-naturally occurring oligonucleotide which does not contain the predetermined target sequence and is complementary to at least a portion of the first oligonucleotide compound. The invention is also directed to synthetic non-naturally occurring oligonucleotide compounds embedded in a tRNA. The ribozymes and compositions of the present invention may be used in vitro or in vivo. They may be used as diagnostic or therapeutic agents. |
申请公布号 |
AU2707295(A) |
申请公布日期 |
1996.01.19 |
申请号 |
AU19950027072 |
申请日期 |
1995.06.21 |
申请人 |
GENE SHEARS PTY. LTD. |
发明人 |
PAUL KEESE;MARIANNE STAPPER;RHONDA PERRIMAN |
分类号 |
C12N15/09;A61K31/70;A61K48/00;A61P31/12;A61P35/00;C12N1/21;C12N5/10;C12N9/00;C12N15/82 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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