发明名称 |
THIOLATION OF PROTEINS FOR RADIONUCLIDE-BASED RADIOIMMUNODETECTION AND RADIOIMMUNOTHERAPY |
摘要 |
A method of radiolabeling a protein with a radionuclide including contacting the protein with a protected tertiary thiol-containing bifunctional chelating agent that is capable of reacting with the protein at one end of the agent and is capable of complexing with a radionuclide at the other end of the agent, to form a protein-acetyl-<u>t</u>-thiol-containing conjugate. The protein-acetyl-<u>t</u>-thiol-containing conjugate then is deprotected and admixed with a reducing agent for the radionuclide, where the radionuclide is added in a subsequent step, to form a mixture of reducing agent and protein-<u>t</u>-thiol-containing conjugate. This mixture then is reacted with a radionuclide whereby the radionuclide reacts with pendant sulfhydryl groups present on the protein-<u>t</u>-thiol-containing conjugate. Methods of radioimmunotherapy and diagnostic kits suitable for forming a composition to be administered to a human patient also are disclosed.
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申请公布号 |
WO9533495(A1) |
申请公布日期 |
1995.12.14 |
申请号 |
WO1995US05539 |
申请日期 |
1995.05.08 |
申请人 |
IMMUNOMEDICS, INC. |
发明人 |
GOVINDAN, SEREGULAM, V.;GREBENAU, RUTH;GRIFFITHS, GARY, L.;HANSEN, HANS, J. |
分类号 |
A61K38/00;A61K39/395;A61K51/00;A61K51/08;A61K51/10;A61P9/08;A61P9/10;A61P31/00;A61P35/00;A61P37/00;C07B59/00;C07K1/13;C07K14/00;C07K16/00;C09K3/00;(IPC1-7):A61K51/08 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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