发明名称 |
NEUE COENZYM UNABHÄNGIGE L-SORBOSONE DEHYDROGENASE. |
摘要 |
PCT No. PCT/EP89/00010 Sec. 371 Date Jun. 11, 1990 Sec. 102(e) Date Jun. 11, 1990 PCT Filed Jan. 9, 1989 PCT Pub. No. WO89/06688 PCT Pub. Date Jul. 27, 1989.A novel coenzyme independent L-sorbosone dehydrogenase originating from a microorganism belonging to the genus Gluconobacter oxydans which acts on L-sorbosone to produce 2-keto-L-gulonic acid. The enzyme has the following physico-chemical properties: a) optimum pH: about 7.0, b) optimum temperature: about 30 DEG C. to about 40 DEG C., c) molecular sturucture: consisting of one type of unit having a molecular weight of about 47,500+/-5,000 as measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis, d) thermostability: stable below 30 DEG C., and e) inhibition: by Cu2+-ions. |
申请公布号 |
DE68924761(D1) |
申请公布日期 |
1995.12.14 |
申请号 |
DE1989624761 |
申请日期 |
1989.01.09 |
申请人 |
F. HOFFMANN-LA ROCHE AG, BASEL, CH |
发明人 |
FUJIWARA, AKIKO, KAWASAKI-SHI, KANAGAWA-KEN, JP;HOSHINO, TATSUO, KANAGAWA-KEN, JP;SHINJOH, MASAKO DAI 3 OHFUNA PARK TOWN G-612, YOKOHAMA-SHI KANAGAWA-KEN, JP |
分类号 |
C12P19/02;C07K14/195;C12N1/21;C12N9/02;C12N9/04;C12N15/00;C12N15/09;C12N15/53;C12N15/74;C12P7/60;C12R1/01 |
主分类号 |
C12P19/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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