Transfection of eukaryotic cells with nucleic acid

摘要

The invention pertains to a method and medium for transfection of higher eukaryotic cells with DNA-polycation complexes wherein the medium contains ethyleneglycol and/or glycerine. In addition, the medium optionally contains a substance that prevents acidification of the endosomes, and/or a lower alcohol such as ethanol. The method is especially suitable for transfection of primary cells such as fibroblasts. This yields stably transfected cells, for example tumor cells to be used as tumor vaccines.