PRODUCING COPIES OF NUCLEIC ACIDS

摘要

Amplification of nucleic acids using oligonucleotides immobilised on solid phase supports is disclosed. Target nucleic acid strands (in a sample to be analysed) are hybridised to the oligonucleotides and copy target strands (incorporating immobilised oligonucleotide) are produced using the target strands as templates. The target strands are denatured from the copy target strands and rehybridised to non-extended oligonucleotides. In the next step, new copy target (1) strands are synthesised from the oligonucleotides hybridised to the target strands, and simultaneously copy target (2) strands are synthesised using previously generated copy target (1) strands. Sequence of denaturing, rehybridising, and producing copy target (1) and copy target (2) strands is repeated as often as necessary to give the desired degree of amplification.