Verfahren zur optischen Bestimmung von Bilirubin und Reagenz dafür.

摘要

A method for optical measurement of bilirubin which comprises reacting a bilirubin-containing sample with a zinc compound, a coloring agent and bilirubin oxidase in a buffer, wherein a stable green pigment having a large molecular extinction coefficient is formed by the action of the zinc compound and thereby bilirubin in the sample can be measured specifically, and a reagent useful therefor. Said method and the reagent are useful for clinical test of total bilirubin and direct bilirubin in serum.