发明名称 |
Hydrogen bond labeling and base sequence determination methods for DNA or RNA |
摘要 |
A hydrogen bond labeling and base sequence determination method for DNA or RNA takes advantage of the complementary nature of the fundamental structure of DNA or RNA bases to bind corresponding chemical species with the base species of a denatured DNA or RNA chain. After providing an aqueous solution containing a nucleic acid, heating the aqueous solution to cleave the nucleic acid; cooling the aqueous solution and adding a base-specific labeling molecule having available hydrogen bonds to the aqueous solution, the base-specific labeling molecules bond to the available bases in a one-to-one fashion via the available hydrogen bonds. The resulting labeled molecule can be microscopically observed to determine the base sequence of the nucleic acid.
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申请公布号 |
US5470707(A) |
申请公布日期 |
1995.11.28 |
申请号 |
US19930025997 |
申请日期 |
1993.03.04 |
申请人 |
HITACHI, LTD. |
发明人 |
SASAKI, YUJI;HARADA, YOSHINORI |
分类号 |
G01Q30/10;C12Q1/68;G01N37/00;G01Q30/12;G01Q60/10;G01Q60/24;G01Q80/00;(IPC1-7):C12Q1/68;C07H21/00 |
主分类号 |
G01Q30/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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