发明名称 |
HEAT CYCLING SYSTEM FOR CONDUCTING POLYMERASE CHAIN REACTION PROTOCOL |
摘要 |
There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme. |
申请公布号 |
JPH07303468(A) |
申请公布日期 |
1995.11.21 |
申请号 |
JP19940303979 |
申请日期 |
1994.12.07 |
申请人 |
PERKIN ELMER CORP:THE |
发明人 |
RARII JIEIMUZU JIYONSON;JIYOUJIFU TOOMASU UIDOUNASU;RICHIYAADO ARAN RIISU;TEIMOSHII JIEI UENBERUGU;RUISU MAIKERU MIZEI |
分类号 |
C12N15/09;B01J19/00;B01L7/00;C07H21/00;C07H21/02;C07H21/04;C12M1/00;C12N15/00;C12Q1/68;C40B40/06;C40B60/14;G01N33/50 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|