| 摘要 |
Methods and apparatus determine analyte concentradon in vivo and in vitro by the steady state determination of luminescence lifetime. A fluoro-phore that is quenched by the analyte is free to undergo Brownian rotation. The fluoro-phore (12) is irradiated with continuous linearly polarized light (24). Emitted luminescence is resolved into vector components parallel and perpendicular to the plane of polarization of the excitation light (36, 38). A mathematical function is employed which relates the luminescence anisotropy to quencher concentration. For analytes which do not quench excited staves, a known quantity of the analyte is conjugated to a quencher molecule or energy transfer acceptor, and a competition reaction is set up in which labelled and unlabelled analytes compete for sites on a labelled carrier molecule. An empirically determined mathematical function is employed which relates luminescence anisotropy at the carrier label emission band to analyte concentration.
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