| 摘要 |
<p>A biosensor for direct analysis of nucleic acid hybridization by use of an optical fiber is disclosed. Single-stranded nucleic acid probes are immobilized onto the surface of optical fibers and undergo hybridization with complementary single-stranded nucleic acids introduced into the local environment of the sensor. Hybridization events are detected by the use of fluorescent compounds which intercalate into double-stranded nucleic acids. The invention finds uses in detection and screening of genetic disorders, viruses, and pathogenic microorganisms. Biotechnology applications include monitoring of cell cultures and gene expression. The invention includes biosensors systems in which fluorescent molecules are tethered to immobilized single-stranded nucleic acid. The preferred method for immobilization of single-stranded nucleic acids is by in situ solid phase nucleic acid synthesis.</p> |
