| 摘要 |
<p>The proposed method involves taking a peripheral blood sample; preparation of a suspension of mononuclear cells which are divided into two equal fractions; cultivation of one of the mononuclear cell fractions without a suppressor cell activator and of the second fraction with a suppressor cell activator (trophoblastic beta-1 glycoprotein); washing of the mononuclear cells to remove the culture medium and blocking of proliferation; addition to each mononuclear cell fraction of freshly separated mononuclear cells taken from a healthy donor and stimulated by the action of phytohaemagglutinin to obtain a test culture; culturing of the mononuclear cells; subsequent assessment of proliferation and determination of the suppression value in relation to the proliferation levels in the test cultures.</p> |
