发明名称 |
TARGETED CLEAVAGE OF RNA USING RIBONUCLEASE P TARGETING AND CLEAVAGE SEQUENCES |
摘要 |
It has been discovered that any RNA can be targeted for cleavage by RNase P from prokaryotic or eukaryotic cells using a suitably designed oligonucleotide ("external guide sequence", or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. An EGS can be modified both by changes in sequence and by chemical modifications to the nucleotides. The EGS can be a separate molecule or can be combined with an RNase P catalytic RNA sequence to form a single oligonucleotide molecule ("RNase P internal guide sequence" or RIGS). Methods are also disclosed to randomly select and to express a suitable EGS or RIGS in vivo to make a selected RNA a target for cleavage by a host cell RNase P or introduced RIGS, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease- or disorder-causing genes in vivo.
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申请公布号 |
CA2185117(A1) |
申请公布日期 |
1995.09.14 |
申请号 |
CA19952185117 |
申请日期 |
1995.03.07 |
申请人 |
YALE UNIVERSITY |
发明人 |
YUAN, YAN;GUERRIER-TAKADA, CECILIA;ALTMAN, SIDNEY;LIU, FENYONG |
分类号 |
C12N15/09;A61K9/127;A61K31/70;A61K35/76;A61K38/00;A61K47/48;A61K48/00;A61P31/12;A61P35/02;C07H21/02;C07H21/04;C12N9/22;C12N15/113;C12Q1/68;(IPC1-7):C12N15/55;C07H21/00;C12N15/11;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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