发明名称 |
Amplification assay for hydrolase enzymes |
摘要 |
Hydrolase enzymes are sensitively determined using novel substituted FAD substrates. The substituted FAD substrates are hydrolysed to FAD by the enzyme to be detected. The FAD is then combined with an apoenzyme to form a holoenzyme which is used to initiate a reaction that leads to a detectable product. When the hydrolase to be detected is phosphatase, the novel substrate is a phosphorylated derivative of FAD. A suitable apoenzyme is apo-glucose oxidase which provides exceptional sensitivity. Apo-D-amino acid oxidase, which is suitable for use in a "single pot" assay system, can also be used.
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申请公布号 |
US5445942(A) |
申请公布日期 |
1995.08.29 |
申请号 |
US19930015287 |
申请日期 |
1993.01.13 |
申请人 |
LONDON BIOTECHNOLOGY LIMITED |
发明人 |
RABIN, BRIAN R.;HARBRON, STUART;EGGELTE, HENDRIKUS J.;HOLLAWAY, DECEASED, MICHAEL R.;HOLLOWAY, LEGAL REPRESENTATIVE, ANN |
分类号 |
C07H19/207;C12Q1/26;C12Q1/34;C12Q1/42;G01N33/533;G01N33/542;(IPC1-7):C12Q1/34;C12Q1/28;C07H19/06 |
主分类号 |
C07H19/207 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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