发明名称 |
NUCLEOSIDE ANALOGUE METHOD |
摘要 |
PCT No. PCT/GB94/02630 Sec. 371 Date May 24, 1996 Sec. 102(e) Date May 24, 1996 PCT Filed Dec. 1, 1994 PCT Pub. No. WO95/15395 PCT Pub. Date Jun. 8, 1995A method is provided of making a dideoxynucleoside mono-or tri-phosphate, which is optionally 32-P or 33-P or 35-S radiolabelled which involves reacting the corresponding dideoxynucleoside with an optionally radiolabelled nucleotide phosphate or thiophosphate donor in the presence of a kinase of phosphotransferase enzyme which catalyses the reaction. A method of sequencing nucleic acids by a chain termination of a kinase or phosphotransferase enzyme which catalyses the reaction. A method of sequencing nucleic acids by a chain termination technique involves detecting the products of enzymatic synthesis by means of isotopically labelled chain terminating nucleotide analogues. |
申请公布号 |
WO9515395(A3) |
申请公布日期 |
1995.07.27 |
申请号 |
WO1994GB02630 |
申请日期 |
1994.12.01 |
申请人 |
AMERSHAM INTERNATIONAL PLC;SMITH, CLIFFORD;FULLER, CARL |
发明人 |
SMITH, CLIFFORD;FULLER, CARL |
分类号 |
C12N15/09;C12P19/30;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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