DNA CODING FUSION PROTEIN OF CATTLE PLAGUE VIRUS

摘要

PURPOSE:To obtain a new DNA useful e.g. for the production of the antigen protein of cattle plague virus for a vaccine against cattle plague virus. CONSTITUTION:This DNA has a molecular weight of 2359 bp and codes a fusion protein of cattle plague virus having the amino acid sequence of the formula as essential amino acid sequence. A cDNA library is prepared from mRNA extracted from the cultured product of cattle plague virus L strain (L13 strain) acclimatized to enable the proliferation in Vero cell. A clone [E.coli DH-RVF-2 (FERM BP-1318)] hybridized with an F gene probe (cdv-F-cDNA) of canine distemper virus is selected from the cDNA and a cDNA insertion part (about 2.4 kb) in a plasmid pDH-RVF-2 in the E.coli is obtained from the clone.