发明名称 |
ADENOVIRUS GENE EXPRESSION SYSTEM |
摘要 |
The present invention provides a novel, highly efficient, recombinant adenovirus expression system for expression of a heterologous gene(s) and/or gene product(s) in a mammalian cell. The recombinant adenovirus was produced by cotransfecting a novel vector with the large fragment of the adenovirus-5 genome in 293 cells. Homologous recombination between these two DNA fragments resulted in the production of the recombinant adenovirus expression system. This vector, when converted to a recombinant virus has the unique capability of expressing one or more heterologous genes at very high levels. The novel vector, comprises, at least one cDNA insertion site for cloning a selected heterologous gene; a promoter sequence positioned upstream from the gene insertion site; the left end replication and packaging elements of the adenovirus-5 genome positioned upstream of the promoter; a highly efficient eukaryotic splice acceptor and splice donor site positioned immediately downstream of the promoter; and positioned downstream of the insertion site a strong polyadenylation sequence and the region for homologous recombination containing a portion of the adenovirus-5 genome. Between the packaging sequence and the CMV promoter are restriction sites for insertion of a second fully functional transcription unit.
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申请公布号 |
WO9516772(A1) |
申请公布日期 |
1995.06.22 |
申请号 |
WO1994US14502 |
申请日期 |
1994.12.14 |
申请人 |
CORNELL RESEARCH FOUNDATION, INC.;FALCK-PEDERSEN, ERIK, S. |
发明人 |
FALCK-PEDERSEN, ERIK, S. |
分类号 |
C07K14/57;C07K14/72;C12N15/861;(IPC1-7):C12N5/10;C12N15/63;C12N15/86;C12P21/00 |
主分类号 |
C07K14/57 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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