| 摘要 |
The invention is a method for reproducing coniferous trees by somatic embryogenesis using plant tissue culture techniques in a multistage culturing process. A suitable explant, typically the fertilized embryo excised from an immature seed, is first cultured on a medium that induces multiple early stage proembryos. These are multiplied in a second culture having reduced growth hormones. Maltose is supplied as the carbon and energy source in the second culture. The early stage embryos grow in size and vigor to advanced early stage embryos. The embryos are then transferred to a cotyledonary embryo development culture. After several weeks somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to soil for further growth. Maltose used in the maintenance and multiplication culture results in larger and more robust advanced early stage embryos which, in turn, produce cotyledonary embryos very similar in morphology to natural zygotic embryos. The use of maltose at earlier stages of embryo development is more important that its use for embryo maturation. |
