NUCLEOSIDE ANALOGUE METHOD

摘要

A method of making a dideoxynucleoside mono- or tri-phosphate, which is optionally 32-P or 33-P or 35-5 radiolabelled, involves reacting the corresponding dideoxynucleoside with an optionally radiolabelled nucleotide phosphate or thiophosphate donor in the presence of a kinase or phosphotranferase enzyme which catalyses the reaction. A method of sequencing nucleic acids by a chain termination technique involves detecting the products of enzymatic synthesis by means of isotopically labelled chain terminating nucleotide analogues.