Enzyme Purification Process

摘要

1,179,523. Purifying enzymes. CHAS. PFIZER & CO. Inc. 18 Jan., 1968 [6 Nov., 1967], No. 2883/68. Heading C3H. A process for purifying and concentrating the mildly proteolytic, milk-curdling enzyme produced by Endothia parasitica or the proteolytic enzyme produced by Bacillus subtilis comprises exposing a solution of the contaminated enzyme to one face of a semi-permeable membrane through which substantial amounts of the enzyme will not pass and through which substantial amounts of solvent, inorganic salts and organic compounds of low molecular weight will pass, the enzyme solution being under a pressure sufficient to cause the solvent to flow from the solution through the membrane, whereby the enzyme is retained by the membrane and the solvents inorganic salts and organic compounds are not. The solvent may be water, and suitable membranes include cellulose acetate and polyelectrolyte types containing polyvinylbenzenetrimethylammonium cationic groups; pressures of 30 to 400 Ib./in.2 are mentioned.